Coding

Part:BBa_K3002000:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-09)


Spectinomycin Resistance for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 586
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

The sequence originates from Enterococcus faecalis and was provided by the MoClo toolkit (pCM0-076-aadA_(SpecR-CDS_B3-B5)). (Weber, E. et al, 2011)

References